Introduction
Diaslide® Urine Culture Device (UCD) is a unique, semi-quantitative culture method for the detection of urinary tract infections demonstrating bacterial counts and isolated colonies. Diaslide® consists of a hinged plastic case containing two opposing agar media (CLED and MacConkey) separated by a sampler with a handle at one end, and two bent sampler prongs at the opposite end.
Following incubation, the number of bacterial colonies is compared with the Colony Density Chart to determine the colony forming units per ml (CFU/ml) of bacteria in the urine sample.
Diaslide® DS-101 UCD) contains two types of media: CLED and MacConkey agar.
CLED’s electrolyte deficiency inhibits swarming of Proteus which otherwise would obscure the observation of colonies. Lactose is included in the agar to detect lactose fermenting coliform contaminants which are easily recognized by the green to yellow color change of the agar.
MACCONKEY agar is a selective and differential medium giving an excellent differentiation between coliforms and non-lactose fermenters of Gram-negative rods with inhibition of Gram-positive cocci. Differentiation of enteric microorganisms is achieved by the combination of lactose and the neutral red indicator. Colorless or pink to red colonies are produced depending upon the ability of the isolate to ferment lactose.
Catalog #DS-101
