Introduction
Diaslide® Urine Culture Device is a unique, semi-quantitative culture method for the detection of urinary tract infections demonstrating bacterial counts and isolated colonies.
Diaslide® consist of a hinged plastic case containing two opposing agar media (UTIselective and MacConkey), separated by a sampler with a handle at one end and two bent sampler prongs at the opposite end.
Following incubation, the number of bacterial colonies is compared with the Colony Density Chart to determine the colony forming units per ml (CFU/ml) of bacteria in the urine sample.
Diaslide® contains two types of media: chromogenic agar UTI selective agar and MacConkey agar.
UTI selective agar is a non-selective chromogenic agar medium composed of a rich nutrient base for isolation and counting of all urinary tract organisms and direct identification via demonstration of enzyme activities of the bacteria most often responsible for urinary tract infections. These include Escherichia coli, Proteus, and Enterococci. The device also provides orientation for the diagnosis of the other urinary pathogens, in particular K.E.S. group Enterobacteriaceae (Klebsiellae, Enterobacter, Serratia and Citrobacter species).
MACCONKEY agar is a selective and differential medium giving an excellent differentiation between coliforms and non-lactose fermenters of Gram-negative rods with inhibition of Gram-positive cocci. Most urinary tract pathogens grow on the medium, whereas most contaminants are inhibited. Differentiation of enteric microorganisms is achieved by the combination of lactose and the neutral red indicator. Colorless or pink to red-colonies are produced depending upon the ability of the isolate to ferment lactose.
Cat# DS-105
